When we are checking antibacterial activity by using nano-particles with reference to antibiotic by using any methods (MIB, MBC, MFC), How confidently we can justify that nano - particles are showing good activity.
Thanks
By obtaining the zone of inhibition at least concentration, we can say the sample shows antibacterial potential.
Respected Dhana Lakshmi
Thank You for your answer, But we know the concentration of antibiotic , but we do not know the concentration of nano-particles, than how can we justify that our nano-particles are better than antibiotic.
Thanks
Concentrate, the preparation of nano sample (0.5mM.1mM, 2mM etc). In the part of AMA, use the Control as alone Nano preparation, Experimental sample (plant or microbial sample) and Finally use nano synthesized sample. ten you can justify wheter Nano or Experimental sample or synthesized sample based on zone of inhibition.
Hai Dhinesh Sir
Thank you for your answer, i did not understand your concept. Sorry for the same
For Example : If i have Pet.Ether Extract with nanoparticles how we can justify our nano particles are better because pet ether it self is toxic.
Thanks
You have to go for toxicology studies or else to minimize the Pet.Ether concentration.
Many of the nanoparticles are toxic but that is based on the usage. When it cross the limit, it leads to defectiveness otherwise GOOD. once again, everything comes under the concentration of sample (see my 1st ans). I hope, u ll get something.
All is well, still if you have doubt on your question just evaluate the nano partical with raw metal (In this situation as possible as to get neither the metal nor nano partical is responsible for the activity)
go to our website www.clemente-associates.com and review the data on silver magnetic particles
Hi Anil,
I suggest either standard plating assays (agar will depend on bacterial genus) or flow cytometry in tandem with nucleic acid stains. I would avoid xtt or a similar metabolic assay as the nanopaeticles may interfere with the readout
Respected Harry c Pappas
Thank you for your answer. Its quite interesting. As we have no facilities of above mentioned assay may be i can not perform. If possible kindly share some papers regarding the same.
Thank you very much for your understanding
Anil,
I suggest taking a look at the attached publication, which is highly cited. Pay particular attention to their method of testing "bacterial susceptibility to nanosilver." I would actually modify their technique, however. It appears that they directly integrate the nanosilver into nutrient agar, and then attempt to grow bacteria on those plates. Instead, I would suggest suspending the cells in an 0.85% NaCl buffer, and then adding the nanoparticles to that at the desired concentration (final concentration should probably be between 0.1 and 100 ug/mL). After a determined incubation period (at room temperature), take an aliquot of that suspension and determine the resulting viability based on colony formation on traditional agar plates.
Best of luck!
Article Does the Antibacterial Activity of Silver Nanoparticles Depe...
Respected Sir Henry C pappas
Excellent Method suggested sir, Thank you so much. I highly obliged to you.
Thank you
Bonjour,
Aprés un essis sur boites de Petri prélever a partir des zones d'inhibition est ensemencer sur milieu Mueller-Hinton vierge. si il n'ya pas de culture aprés 24 heures d'incubation c'est que votre nanoparticul est efficace et du même cout vous avez les CMI
After a test on Petri dish withdraw from zones of inhibition is sowing on Virgin Mueller-Hinton medium. If there is no culture after 24 hours of incubation is your nanoparticles is effective and the same cost you have the CMI
plz find the link i attached. we made it with comparison the count of bacteria with and without inoculation of them to nano-silver in broth medium.
http://nsft.sbmu.ac.ir/browse.php?a_id=1498&sid=1&slc_lang=en
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