30 April 2021 1 5K Report

Isolation of protozoans from water samples

More Sandra Gogo Okere's questions See All
How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

In silico techniques for identification of inhibitor/activator/substrate activity?

Hello dear researchers, I would like to know about available in silico tests that could predict the activity of ligands (small molecules) against the target protein. I have performed docking...

08 July 2024 4,724 4 View

Any new data on DMT1/2 Covid-19 Omicron Survival?

Still alive but new info from last hospitalization a week ago. Per Endo ... Covid remapped the electrolyte panel. A high potassium 5.5 actually is low and the patient needs K+ Blood glucose at...

21 May 2024 4,780 0 View

Does anyone have experience with studying GSK2881078 on muscle cells?

The past few months I have started testing the selective androgen receptor modulator (SARM) GSK2881078 to study its ability to increase muscle growth and/or counteract muscle atrophy. I have done...

20 May 2024 8,747 0 View

Can hydrogen peroxide stimulate magrophages for the induction of nitric oxide?

Can hydrogen peroxide stimulate magrophages for the induction of nitric oxide? What is the protocol?

16 April 2024 1,409 1 View

Is it necessary to use a normalization microRNA in each qPCR plate?

For validation by qPCR of several microRNAs, is it necessary to measure normalization miRNAs in each plate in addition to the miRNAs under study? Or can you make plates of microRNAs and then...

15 April 2024 6,393 1 View

How can I make a baseline correction of a FT-IR?

Hello everybody, I've been trying to fix the FTIR attached to this enquiry but I havent been lucky at all. I would like to know if anyone would be so kind as to help me fix this spectrum or tell...

25 March 2024 7,648 0 View

Cómo se hace los "Traits en PopArt?

I need a little help, how do you make "Traits" in PopArt? I need to make a network of haplotypes on a map

14 March 2024 2,316 0 View

Protein modeling programs with a free commercial license?

Hello, I wanted to ask for help with protein modeling programs (not servers) with a free commercial license. thank you

13 March 2024 6,173 0 View

How to make a 33bp deletion from a plasmid?

I would to delete up to 33 bp from a plasmid to generate deletion mutation. Has anyone tried using Agilent Quikchange lightning site direct mutagenesis to delete such a long region? How feasible...

06 March 2024 8,136 5 View

Similar questions and discussions
• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

Is it true that $\det(V(A))$ may be only $\pm 1$, depending on $n$, for the last symmetric tridiagonal matrix $A$?

One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...

08 August 2024 6,690 1 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close