Lactic acid bacteria (LAB) can be isolated from traditional pickles by appropriate dilutions with saline, plating on MRS (de Mann Rogosa Sharpe) agar supplemented with 1% CaCO3 to distinguish the acid-producing bacteria from other bacteria and then incubating anaerobically at 30 °C for 48 h. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5495720/
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