I want to isolate DspE from Erwinia amylovora. Please suggest me the procedure which required to isolate the DspE Protein. I don't want any cloning experiments.
1- Grow Erwinia amylovora cells in an hrp gene-inducing minimal medium (Science 345:1374–1377).
2- Perform 2D Gel with a protein ladder in 2nd run, at about 173KDa (http://web.expasy.org/cgi-bin/compute_pi/pi_tool1?D5GSK5@noft@average) is your protein of ineterest.