The double log graph of Stern-volmer equation is showing is best fitting with exponential increment. How can I explain it? what is its mathematical expression and interpretation? the linear equation is log[F0-F]/F= n log[Q]+logK. it gives the results like n (number of binding site) and K(Binding constant). What information the exponential graph will give?
Dear Priyankar,
There is some thing wrong with these plots. Stern-Volmer plots for dynamic fluorescence quenching (DFQ) are not double log plots. I am enclosing 3 papers. Kindly replot accordingly. Kindly keep in mind Stern-Volmer plots can also be Biphasic (see attached papers).
F/F0 = 1 + Ksv [Q] ........... see attached paper (Cavicchioli et al. 2006).
A plot of F/F0 versus acrylamide concentration [Q] gives a linear plot of slope Ksv (stern-volmer constant).
Best wishes
Sohail
Stern Volmer double log plots are new to me. Do you have Reference
for these plots as i am interested?
thanks
sure.
Inter-domain helix h10DOMI–h1DOMII is important in the molecular interaction of bovine serum albumin with curcumin: spectroscopic and computational analysis
D Pangeni, C Kapil, MA Jairajpuri, P Sen
European Biophysics Journal 44 (3), 139-148
Interactions of thioflavin T with serum albumins: spectroscopic analyses
P Sen, S Fatima, B Ahmad, RH Khan
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 74 (1 ...
Dear Priyankar,
Were you able to solve the problem. In my case, I am getting linear stern volmer plot but exponential double log graph.
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