How to prepare FeEDTA?

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How can I interpret double log quenching equation when graph shows exponential change?

The double log graph of Stern-volmer equation is showing is best fitting with exponential increment. How can I explain it? what is its mathematical expression and interpretation? the linear...

03 April 2016 8,603 6 View

What are the factors taken care of during purification of unstable proteins?

Theoretically, my protein is unstable. till DNA level everything is quite fine, but I think I am losing it during heating with bromophenol for SDS-PAGE. Any suggestion?

03 April 2016 5,225 5 View

Are there any prediction tools for inorganic ligand and solvent interaction?

I am searching an online tool (preferably free of cost) which can theoretically predict interaction (thermodynamic parameters, involving functional groups) of a molecule (in my case its an...

31 December 2014 356 1 View

Is anyone familiar with polyproline tags for purification?

Hello friends, I have a protein (without his-tag) naturally having one pentaproline sequence in the solvent accessible position. Does anybody have experience with purification using the help of...

10 November 2014 2,044 1 View

Why do I observe multiple peaks in flow-through?

I am trying to purify one protein in 2mL agarose-NTA-Ni2+ column at flow rate less than 1mL/min (equilibrated in 100mM phosphate buffer pH 7.4 with 1M NaCl). Multiple peaks can be observed in...

10 November 2014 6,443 10 View

Can start site be predicted?

The interest of my DNA sequence has 11 codons for methionine. Can I predict from which one protein synthesis will start?

01 February 2013 4,691 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,469 1 View

Can I separate xylene from paraffin after deparaffinization of microtome samples and reuse the xylene?

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24 July 2024 1,559 1 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Do you know CUSABIO Biotechnology Scholarships for Students 2024?

I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...

22 July 2024 5,790 1 View