What are the factors taken care of during purification of unstable proteins?

Theoretically, my protein is unstable. till DNA level everything is quite fine, but I think I am losing it during heating with bromophenol for SDS-PAGE. Any suggestion?

03 April 2016 5,136 5 View

How can I interpret double log quenching equation when graph shows exponential change?

03 April 2016 8,497 6 View

Are there any prediction tools for inorganic ligand and solvent interaction?

I am searching an online tool (preferably free of cost) which can theoretically predict interaction (thermodynamic parameters, involving functional groups) of a molecule (in my case its an...

31 December 2014 269 1 View

Is anyone familiar with polyproline tags for purification?

Hello friends, I have a protein (without his-tag) naturally having one pentaproline sequence in the solvent accessible position. Does anybody have experience with purification using the help of...

10 November 2014 1,943 1 View

Why do I observe multiple peaks in flow-through?

I am trying to purify one protein in 2mL agarose-NTA-Ni2+ column at flow rate less than 1mL/min (equilibrated in 100mM phosphate buffer pH 7.4 with 1M NaCl). Multiple peaks can be observed in...

10 November 2014 6,340 10 View

Can start site be predicted?

The interest of my DNA sequence has 11 codons for methionine. Can I predict from which one protein synthesis will start?

01 February 2013 4,592 4 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View