Priyankar Sen

7 Questions 38 Answers 0 Followers

Questions related from Priyankar Sen

How can I interpret double log quenching equation when graph shows exponential change?

The double log graph of Stern-volmer equation is showing is best fitting with exponential increment. How can I explain it? what is its mathematical expression and interpretation? the linear...

04 April 2016 8,565 6 View

What are the factors taken care of during purification of unstable proteins?

Theoretically, my protein is unstable. till DNA level everything is quite fine, but I think I am losing it during heating with bromophenol for SDS-PAGE. Any suggestion?

04 April 2016 5,203 5 View

Are there any prediction tools for inorganic ligand and solvent interaction?

I am searching an online tool (preferably free of cost) which can theoretically predict interaction (thermodynamic parameters, involving functional groups) of a molecule (in my case its an...

01 January 2015 334 1 View

Why do I observe multiple peaks in flow-through?

I am trying to purify one protein in 2mL agarose-NTA-Ni2+ column at flow rate less than 1mL/min (equilibrated in 100mM phosphate buffer pH 7.4 with 1M NaCl). Multiple peaks can be observed in...

11 November 2014 6,412 10 View

Is anyone familiar with polyproline tags for purification?

Hello friends, I have a protein (without his-tag) naturally having one pentaproline sequence in the solvent accessible position. Does anybody have experience with purification using the help of...

11 November 2014 2,014 1 View

How to prepare FeEDTA?

How to prepare FeEDTA by adding Feso4 7h2o and NaEDTA, avoiding precipitation?

02 February 2014 4,512 3 View

Can start site be predicted?

The interest of my DNA sequence has 11 codons for methionine. Can I predict from which one protein synthesis will start?

02 February 2013 4,657 4 View