Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

Do you consider it is important to vindicate Spanish as language, in scientific issues and publication of scientific articles?

03 February 2021 3,756 4 View

Do we need to keep looking for the fracture angle after the Puck's failure initiation is reached?

Greetings to everyone, Our team is currently working on the development of a VUMAT code in Abaqus for composite modelling using the Puck's failure theory as damage initiation criteria. Currently...

20 January 2021 6,367 1 View

IC50 for plant extracts?

If I am working with plant extracts and their photoprotective properties.... It is just enough to evaluate the citotoxicity at a specific (high) concentration of my extracts or it is necessary to...

14 September 2020 8,890 1 View

How to do a list of references by chapters with Mendeley?

Hi everyone. I am writing the manuscript of my thesis, and I would like to make a list of references by chapter. I mean that every first reference of each chapter begins for [1] using the IEEE...

10 September 2020 524 3 View

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Online data repositories are useful tools that ensure the reproducibility and repeatability of scientific investigations. For the sake of facilitating collaboration between researchers in the...

31 August 2020 4,826 3 View

Statistical analysis of hunting sequence, animal behaviour or ethogram?

Hi Everyone, I am working on analyzing a hunting sequences from different animals. I have written down each behaviour per second and have them as categorical data in order of appearance. An...

28 April 2020 4,007 7 View

How do Pierce Strong Ion Exchange Spin Columns work for protein purification in place of using FPLC?

I have used an Ni-NTA system for purify five different his tagged proteins produced in E.coli , optimizing the amount of imidazole for the equilibration, washing and elution steps however the...

31 March 2020 2,774 5 View

How delete a file that just upload, to include abstract?

I forget to include the abstract and I would like to delete the file of my book and replace for a new one

30 March 2020 7,688 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View