Hi everyone
I am working on a rat model of uremia with high adenine feeding. The aim is to induce arterial calcification using this model. From the literature I have gathered, it seems to take a time of 8-10 weeks for the calcification to be initiated. We need it to be sooner than that. Have any one of your worked on this model before and how were your results?
There is one publication by Paul Price and his group which modifies the diets to achieve calcification earlier. Other groups have used this model ad libitum. Has anyone used this model before? What are the various ways to ameriolate mortality that is caused by this model?
Thanks
Dear colleague,
I don't work in rat uremia model, but in Spain, Mariano Rodriguez-Portillo (from Cordoba) is working on it. He is an expert in calcification in rat models.
Feel free to send him an email to ask your question.
email: [email protected]
I'm really sure that Mariano will answer your question
Regards
Dear Saketh,
we worked quite a bit in Terms of adenine, warfarin and subtotal nephrectomy to induce calcification.
With the Price Papers you are already on the spot. In our Hands Adenin is the most pro-calcifying technique however I feel the System is pretty different from uremic calcification and rather a toxic one with potential direct effects on the vascular System (only some gut Feelings). You may try to set the animals on "low Protein diet" - there are some papers from the Price Group showing that this accelerates calcification.
best
Ralf
Try a high phosphate diet (1.2%) and if that is not enough, adding a calcitriol shot three times a week will work.
I believe that increasing P in the diet is the right solution. I remember that in the Eduardo Lab the model of uremic rats (surgically obtained) was successful when nurished with 1.2% or more. The uremic rats survived for 6 months or longer and this was the basis of the study of Mario Cozzolino (See KI, 2002 (?)) who evaluated vascular and soft tissue calcifications in uremic rats treated with Sevelamer.
Best regards.
Diego
Thank you everyone for your kind replies. They were really helpful. I am currenty testing a modified diet with high Ca and P.