How can I increase concentration of fungal growth in PDB medium?

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Giorgia Pertile

When I culture the fungus in PDB I need to wait 2-3 days before see something on the medium. For the OD for fungi you need to measure at 750 nm and not 600 nm.

Manavi Ekanayake

Fungi take much time to grow (3-5 dys), than the bacteria. Did you try with different shaking conditions (100, 150 rpm)?

Augustine Antwi-Boasiako

Thanks Manavi. I have used 200 rpm for two trial and the results are similar. I have vary the shaking conditions. Thanks.