Dear Sir. Concerning your issue about how to increase adenine solubility in water . Research on nucleobases has always been on the forefront owing to their ever-increasing demand in the pharmaceutical, agricultural, and other industries. The applications, however, became limited due to their poor solubility in water. Recently, ionic liquids (ILs) have emerged as promising solvents for nucleobase dissolution, as they exhibit >100-fold increased solubility compared to water. But the high viscosity of ILs remains as a bottleneck in the field. Here, by solubility and viscosity measurements, we show that addition of low-to-moderate quantity of water preserves the high solubilizing capacity of ILs, while reducing the viscosity of the solution by several folds. To understand the mechanism of nucleobase dissolution, molecular dynamics simulations were carried out, which showed that at low concentrations water incorporates into the IL–nucleobase network without much perturbing of the nucleobase–IL interactions. At higher concentrations, increasing numbers of IL anion–water hydrogen bonds replace IL–nucleobase interactions, which have been confirmed by 1H- and 13C NMR chemical shifts of the IL ions. Based on the fact that adenine (A) is highly soluble in water, especially hot, and with water temperature decreases, its solubility falls10, so adenine solution was prepared by dissolving 0.0135±0.0002 g of its sample in 10 ml of bidistilled water under constant heating. I think the following below links and the attached file may help you in your analysis:

http://pubs.acs.org/doi/full/10.1021/acs.jpcb.5b07179

http://pubs.acs.org/doi/abs/10.1021/bi00775a025?journalCode=bichaw

https://www.ncbi.nlm.nih.gov/pubmed/8008706

Thanks

Adenine is much more soluble in hot water, than in cold water, where is only very slightly soluble. Regarding to lower temperature aqueous solutions, we might want to consider (e.g.): (i) aqueous NH4OH―NH4Cl buffer solution at pH 10; (ii) tert-butanol―water mixed solution, preferably alcohol-rich to improve solubility; (iii) dimethyl sulfoxide (DMSO)―water mixed solution, preferably DMSO-rich.

Thank you very much. I appreciate!

But the aim of this work is to obtain adenine-perrhenate compound in crystalline form (pteferably single crystal). Any advices on which water-another solvent system will be convenient for performing this reaction?

Phosphate buffer

Could you please suggest me how to prepare aqueous solution of adenine (2mM, 8mM) for cell culture?

Jawad Alzeer Have you dissolve adenine or bases in phosphate buffer? Can you please help me to provide with the composition of phosphate buffer which you used? Thanks

Would you elevate the temperature to 60 0C, please?

How long Atakilt Abebe ?