We didn't modify the protocol ( before we didn't have this problem as often )or done nothing different than usual. Any suggestions are most welcome.
I have similar problem now, and I'm afraid that this is contamination. Have you managed your problem?
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...
24 February 2021 2,006 2 View
I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...
21 February 2021 6,046 4 View
My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...
11 February 2021 6,009 3 View
Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance
09 February 2021 717 6 View
Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...
30 January 2021 2,865 4 View
I'm staining some brains with several stemness markers but the OCT4 antibody that I have been using is not working. I would really appreciate your recommendations! Thank you!
25 January 2021 3,169 3 View
Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...
25 January 2021 757 3 View
When we are doing sampling by means of simple systematic random sampling,how do we calculate the design effect for use during calculation of sample size for our work?
20 January 2021 5,328 3 View
Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...
18 January 2021 6,683 1 View
Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...
25 February 2021 8,139 1 View
Hello Everyone! I was looking for the effects of the temperature and the relative humidity on the dropwise condensation. I was looking for the papers related to it explaining it in deep. I looked...
12 February 2021 4,196 2 View
I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...
28 January 2021 3,867 1 View
hello i am trying to study the mechanical deformation due to the humidity on a 3d volume formed of 2 phases. my equation are dw/dt =grad(Dm*div(w)) and the mecanical is sigma=E*beta*nabla(w)....
27 January 2021 5,201 1 View
Properties such as relative humidity (T 402sp-03), brightness and yellowness (T-452 om-02), whiteness (T-560 pm-96), opacity and absorption coefficient (T-425 om-01), tensile, breaking length and...
24 January 2021 308 3 View
I have a box which is kept at 4C which is enclosed in a ziploc bag. The dimensions of the box are: 18cm x 13.4cm x 4.9cm. The relative humidity of this internal environment is 30% at this...
07 January 2021 6,734 3 View
Eleven months ago I girdled 17 trees and measured the moisture content, at the place where they had been girdled, with a wood moisture meter. Typically, the moisture meter showed a reading of...
26 December 2020 7,761 2 View
I am working in the area of organic solar cells. I need to anneal my samples in 1% humidity conditions and we don't have a glove box. How can I set up a chamber for this?. Is there is any...
22 December 2020 6,503 5 View
I have temperature and percipitation data for some years and I would like to examine the thermal comfort of the region. In my knoledge even the calculation of the simpliest indices requires...
20 December 2020 7,207 3 View
I need to design new oligonucleotides that can be used for gRNa/Cas9. The oligonucleotides need to target a gene from melangoster drosophila. Where do I start? How to find the gene to knock...
15 December 2020 4,729 3 View