We didn't modify the protocol ( before we didn't have this problem as often )or done nothing different than usual. Any suggestions are most welcome.
I have similar problem now, and I'm afraid that this is contamination. Have you managed your problem?
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Information literacy in higher education integration with curricula engineering
04 August 2024 5,368 3 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
Please (for Arabidopsis), what could be a good Lumens and color range (Kelvin) range for full spectrum LED lamp tubes size T8 (120cm) for each shelve measuring 130x50 cm (length x width) and 60 cm...
11 July 2024 6,078 1 View
What are the standard applications of Cross Attention in Transformer Architectures ?
09 July 2024 9,310 2 View
Are there standard RNN architectures been applied for Time Series Analysis, forecasting and anomaly detection problems ?
30 June 2024 3,169 8 View
Have we ever used LSTM architectures on Time-Series Forecasting and Analysis, and gotten a decent result ?
30 June 2024 6,924 3 View
I am new to running PCR gels. I loaded this gel and I thought it was fine, meaning I saw/felt no apparent punctures or spillovers to neighboring wells (see picture 1). When the gel started to run,...
30 June 2024 4,107 4 View
Where are large vision models typically used ?
25 June 2024 4,113 0 View
When it comes to Re-inforcement Learning with Human Feedback, are there standard libraries/frameworks for training LLMs ?
25 June 2024 1,121 0 View
How does high humidity affect the growth and development of crops?
10 July 2024 7,258 20 View
Hi, I need to measure the real-time (or temporal intervals) thickness variation of a transparent material from dry to wet/humid conditions, however, I cannot use the following techniques: -...
08 July 2024 4,799 4 View
Hello, I am doing some testing on lateral flow devices/materials and have run into a slight issue where I do not understand what the cause may be, but I have some ideas. General Experimental...
01 July 2024 7,960 3 View
I want to estimate daily potential evapotranspiration using the Penman-Monteith formula. My weather station measures temperature, humidity, wind speed, light intensity, and UV every 15 minutes,...
21 June 2024 5,159 0 View
Hello forum members, I'm conducting research on vegetable oil fingerprinting. I need annual precipitation (rainfall) data for provinces in Thailand, Malaysia, and Indonesia. Specifically, I don't...
30 May 2024 6,209 2 View
kindly please help me to plot MFD using specific humidity +U+V using python.
20 May 2024 8,292 4 View
I have noticed massive media evaporation (>50% volume) from culture dishes after 72-96hr inside a Memmert ICO50 hypoxic incubator (Temp: 37C, humidity: 86%, CO2: 5%, O2: 1%). I've confirmed the...
29 April 2024 9,872 1 View
Pre sowing irrigation was applied, irrigation after 2 days of sowing in clayey soils of terai region of Nepal 🇳🇵,very low RH, hugh wind speed and day temperature avove 40 degree celcius and heat...
23 April 2024 3,943 2 View
Dear all, I have performed CUT&TAG and tried to pulldown H3K27me3 containing nucleosomes in the cells treated with EZH2 inhibitor. I spiked my human cells with 20% of Drosophila cells. As...
21 April 2024 3,197 0 View
Hello team, I am using Mirdeep2 to identify miRNA in my organism (none model organism) which has no previously identified micro_RNAs. My organism is genetically close to Drosophila melanogaster....
14 April 2024 9,090 0 View