in experiment to propagate some olive cultivars through tissue culture technique a low rate of multiplication was observed even with addition of zeatin also the addition of PPV have a negative effect during starting stage. the addition of auxin resulted in production of large amount of callus with a week shoot (less than 2 cm). if any one have an idea about media addition or any other treatments to improve olive micro-propagation?
Hello Abdou
We use the technique developed by Professor Ronald de Fossard which is a series of experimental media with different levels, originally he had 81 experimental media, he then modified this in 2003 to 23. His CD's are available if you google him online.
We stopped wasting time and media once a known formula did not work. It was worth the effort with the stock solutions in the end. Sometimes we focus on the hormones and not the media composistion
Professor de Fossard developed these so that there was a scientific method in developing media protocols and we find this resource invaluable
I hope this is of some assistance and would encourage you to also try the metapolin as a cytokinin too.
Hello Abdou
We use the technique developed by Professor Ronald de Fossard which is a series of experimental media with different levels, originally he had 81 experimental media, he then modified this in 2003 to 23. His CD's are available if you google him online.
We stopped wasting time and media once a known formula did not work. It was worth the effort with the stock solutions in the end. Sometimes we focus on the hormones and not the media composistion
Professor de Fossard developed these so that there was a scientific method in developing media protocols and we find this resource invaluable
I hope this is of some assistance and would encourage you to also try the metapolin as a cytokinin too.
I would not encourage the formation of callus and change for liquid media renewed weekly to remove oxidation
Dear sir,
It is well known than cytokinins promote quick differentiation of shoots from any type of explants, also proven in your experiments. Avoid adding auxins to promote shoot initials formation and quick differentiation of shoot for micropropagation experiments.
Best wishes,
sgdoss
Dear colleague,
I worked a lot to identify a good substrate for olive explants. Frst of all the best carbohydrate for olive is the mannitol, this sugar inhibits the callus formation at the explant base and improve the growth of buds . i send you my publications, moreover you will find a very innovative rooting technique for rooting.
Sincerely yours, Annarita
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