How can I improve the concentration of banana fruits extracted RNA?

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Could someone please provide a list of journals that accept the application of methodologies in nature-based solutions?

I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.

23 July 2024 6,444 2 View

What is the difference between neuropsychology and cognitive neuroscience ?

The main differences

14 July 2024 3,158 4 View

About mineral exploration?

Are there any techniques at ore exploration that could replace the compass-tape measure of mineralized structures?

14 June 2024 3,254 2 View

What is the minimum amount of liquid and beads I can use for bead-beating in a 2ml vial?

I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of...

14 May 2024 2,291 2 View

Can I replace some of the buffers from the first steps of QIAamp Mini kit by DNAshield?

I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of...

14 May 2024 7,178 3 View

How do I measure the virulence intensity of a fungus strain in a host plant?

I need to determinate how virulent is a fungus/oomycete strain-specie in comparative with others. I have plants group with and without fungus symptoms. However I found the fungus sources in both...

06 May 2024 8,974 5 View

Are multinational companies in Papua New Guinea heavily taxed?

I am doing a Case study on the impacts of tax on Multinational companies

26 April 2024 9,598 1 View

What is the answer to this?

On 1st January 2030 Zivick Co. assigned K9,000,000 from their Accounts Receivable of K12,000,000 to BSP under a notification arrangement. BSP bank loans 85% less 1.2% service charge based from...

29 March 2024 8,705 0 View

Portable orbital shaker?

Hi all I need to keep some samples at -35 to -25 degrees on an orbital shaker for a few hours or so. The only way I can think of doing this is to keep the samples a land shaker in a freezer....

17 March 2024 6,228 1 View

Do you know about guides/tutorials for Distance Based Linear Modelling (DistLM) in Statgraphics?

I have to choose the statistical analysis for my thesis proposal, and this statistical analysis was used in a topic similar to mine (to analyse the relationship of some environmental data with...

01 March 2024 388 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View