I'm trying to make recombinant protein, but after digesting my inserts, and purification via NucleoSpin extract kit, I always end up with poor plasmid concentration and very low 260/230 ratio, even though i tried to consider the possible mistakes! and when i continue the transformation with the same DNA, i can see no bands at all on the gel, although the bacterias are growing.

Does anybody had similar experience or have some ideas to help me overcome this problem?

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