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26 December 2020 9,690 3 View

In dissipation engineering. Why the microscopic mechanisms of damping are limited by low frequencies?

I am starting working on damping in metamaterials, and I found the following statement that caught my attention: "Dissipation engineering at the macroscopic level is additive; it builds on what...

05 June 2019 9,566 4 View

How can I improve a DNA extraction from serum?

Hi everyone! I'm working on a DNA extraction from serum. Unfortunately the concentration was very low and the PCR amplification didn't work, maybe because the DNA extracted was very fragmented. Do...

23 August 2018 4,155 3 View

What is the most cost and time effective way to assess the isoprene synthesis capacity of a poplar leaf?

Dimethyl allyl diphosphate (DMADP) is transformed to isoprene and pyrophosphate (PPi) thanks to the isoprene synthase. Is it better to: - measure the concentration of isoprene synthase assuming...

05 June 2018 3,208 2 View

Is it enough an Exonuclease treatment before sequencing?

Hi everyone! I would like to know if an exonuclease I treatment after isolating DNA samples from an agarose gel is enough for sequencing. I know that it is recommended to use ExoSap which...

04 May 2018 5,413 4 View

Can anybody tell what are benefits of using a I Microlabbox Vs a DSPACE DS1104?

I'll buy a DSpace data acquisition system, which will be used to measure current and some other variables in brushless motors. I saw that in many publications the people use the DSpace DS1104....

01 February 2018 6,423 0 View

How Can You Prevent Nanoparticle Agglomeration in Solution?

In trying to store and analyze some iron oxide nanoparticles I find that they agglomerate and so I cannot measure them by DLS. My particles are coated with a polypyrrole polymer shell. Should I...

22 January 2018 6,778 5 View

What buffer should I add to the Krebs-Henseleit solution to counteract the rise of the PH while using pure oxygen instead of carbogen?

I am currently working with the Langendorff's Isolated Heart System and I have some problems with the Krebs-henseleit solution due to a change we were force to do after we ran out of carbogen and...

04 October 2017 9,914 2 View

Why do I get a pink pellet during RNA extraction after isopropanol precipitation and centrifugation?

15 October 2014 7,314 6 View

Can anyone help me setup a particular relative humidity with salts?

Hi, I want to set up 59% of relative humidity, which salt do I have to use? Thank you very much.

06 July 2014 9,396 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View