After getting my cyanobacteria-water samples, filtering them through GF/F filters, and freezing-thawing them, I use the trichromatic method to get Chl-a concentration.
I am wondering how much "free" (not cell-associated) chl-a is passing through the filter? is it possible to measure that portion that stays in the filtrate? If so, how would I do it?
I would try to extract chlorophyll, e.g., into the diethyl ether. Maybe, addition of some salt into the aqueous phase could help, and several extraction will be needed. Next steps are to evaporate ether and redissolve the residue in desired solvent.
Dear Kelly,
the quickest and easiest (but still with high sensitivity) would be using a plankton fluorometer. Most of these will also provide an estimate of phycocyanin and phycoerythrin, which might be useful for your cyanobacteria-related question.
Good luck
Marco
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