Why do molecular orbitals need to be orthogonal in Hartree-Fock?

When deriving the Hartee-Fock method, we minimize the electronic energy with respect to all molecular orbitals with the contraint of orthonormality of the molecular orbitals by using the method of...

09 October 2019 3,805 1 View

Why are more males born than females?

The currently accepted value of the proportion of female births in large European-race populations is 0.485 (Bayesian Data Analysis, 3rd edition, Gelman et al.). Do we know why the probability of...

02 March 2019 7,209 19 View

How can I reduce the stiffness of a system of equations?

I am dealing with a large system of nonlinear equations and I am having trouble computing its zeros numerically because it is very stiff. Can you suggest approaches to deal with this? Perhaps some...

09 October 2018 5,822 6 View

What are the disadvantages and limitations of drug pump devices?

Drug pumps seem an interesting option for managing chronic pain. I have seen similar devices used for insulin delivery. I wanted to know what are the disadvantages, complications, and limitations...

08 September 2018 4,054 3 View

Science and Religion

Religions are an important part of the cultural richness of the society and they should be respected. However, those working in science may face questions about it, as the tenets of some religions...

01 January 1970 7,447 38 View

How to extract the trajectory of a subset of atoms in Gromacs?

Hello, Is there an easy way to generate a new Gromacs trajectory file md_subset.xtc from an existing trajectory file md_allatoms.xtc where I remove everything (including solvent) except a subset...

01 January 1970 2,134 2 View

What would you change in your lab to make it a better place?

I believe that safety and comfort in the lab are fundamental for scientific research. Thus, I would love to hear, based on your experience, what would you have or change in your lab to make it a...

01 January 1970 8,844 5 View

Solving protein structures: X-ray vs NMR

Given the different quality metrics for protein models based on XRD and NMR data, I want to know your opinon. In particular, how good is the agreement between NMR and XRD-derived structures? In...

01 January 1970 3,735 10 View

What is your motivation to do research?

I would love to hear how your scientific motivation and goals have evolved during your career. What motivates you to do science now, and do you feel it has changed over the years? Do you feel...

01 January 1970 8,689 15 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

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01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View