http://www.atsu.edu/faculty/chamberlain/website/lab/idlab/flowchp.htm

http://www.uiweb.uidaho.edu/micro_biology/250/IDFlowcharts.pdf

Thank you so much Jennifer Gibbons mam and Joel Marx sir for your kind considerations and providing me valuable information.

Go for Biochemical and Cultural characterization. First slide may be Bacillus subtilis, second one Bacillus megaterium and last one Bacillus cereus or anthracis. U will get more clarity over species among bacillus genera by 16S rRNA gene sequencing. 

Thank you so much sir for helping me regarding this concern, my work is on keratin degradation and these are the isolated bacteria. I completed biochemical tests of these bacteria. Now I sent them for 16s r RNA gene sequencing.

After getting the  nucleotide sequence u need to gor BLAST using NCBI data base. 

one more question to respected Krishna sir,  please tell me the name of research lab/institution/company for 16s r RNA sequencing in India.

Go through the following web sites. nccs-mcc pune, or ncl-ncim or mtcc. U can get web address through google. Among all sites jus go through identification services option. 

You can go for 16S rRNA gene based analysis of these isolates which will give you clear idea about the identity of the isolates.

Thank you so much Krishna Rayudu sir and Avinash Sharma sir for your kind considerations. I search the NCCS-MCC pune weppage and IMTECH for !6s rRNA sequencing and the charges are above 10,000 per sample.

Do the partial 16rDNA sequencing and check your results from the phylogenetic tree of BLAST.