It is hard to say without seeing the quality of the original .abi sequencing files but often primers for bacteria are multiply degenerate so will amplify many species so there may be a mixture of species present which might be indicated by the number of polymorphic bases in the sequence .It would be interesting to see the sequences.

Dear Gamal Abdel-Aziz

Se your sequence in e.g. FinchTV. It is free online tool. Maybe, some nucleotides were not identified by sequencer software but you can edit them based on colour of the peak. As @Paul Rutland sugested, the quality of sequence is crutial. Also, you can concatenate your forward and reverse sequences into one longer fragment, which could provide you better result in BLAST search.

If the quality of your sequence is good, then use the DNABaser software to assemble your forward and reverse sequences into a contig. Download the EZBiocloud identification service and use the contig to get the closest match of your sequence.

Paul Rutland Dear Prof

thanks for your reply, how to judge on the .abi sequence, is this good or not.

i can send you the files for review it , if your time permit

and please how to say it is new strain or not.

best regards

Marcela Krutova thanks so much Professor

will make with your advice

and also hope for scientific cooperation if it possible

Best regards

Gamal

Please do send the sequence files Gamal Abdelaziz I would be most interested in seeing them. It is important that the sequencing comes from only one colony to stand any chance of being uniquely identified so it is easier to show that a sequence is derived from more than one species and there are many others on this website more qualified than I am to assess the novelty of the sequence but it would be interesting to see