How can I get rid of some unspecific haemolytic substance in seed extract that has blood group specific lectin?

21 December 2012 1 3K Report

How can I get rid off some unspecific haemolytic substance in seed extract that has blood group specific lectin? The haemolytic property is neutralysed by adding bovine serum albumin, though but it is undesirable contamination to make it as reagent.

Steingrimur Stefansson

There are some good suggestions provided to a similar question posed by Thierry Rolling on Dec. 20.

Also, if the hemolytic property binds to serum albumin you can try depleting it from your extract by passing it over immobilized albumin.

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