Can You predict the estimated fruit size of a cultivar from average leaf area?

More Ákos Mendel's questions See All

Why is Fc tag only present on a fraction of my produced recombinant proteins?

Hi! I have trouble finding literature on this. Can someone please share some information on what could be the cause of a glycoprotein-Fc-tag fusion protein being expressed in HEK cells missing its...

19 October 2021 3,080 3 View

How much poly-A RNA should I use for transcriptome sequencing using minION (ONT)?

I extracted ~87 ug of total RNA from mushroom samples using TRIzol (Invitrogen) and I got ~1,2 ug of poly-A RNA using the Oligotex kit (QIAGEN) for mRNA purification. The protocol of direct cDNA...

31 March 2021 2,063 0 View

Any hint on adding a phylogenetic tree tip labels in MEGA X or FigTree 1.4.4 ?

Hello, how do I make a neat phylogenetic tree, such as the one you can see in the attachment (Fig1.jpg) ? Notice how there are multiple taxa displayed under each other on a single terminal branch...

25 March 2021 9,772 3 View

Why degrade my cDNA during the second strand synthesis reaction?

Dear All, I would like to ask some help in second strand synthesis. I’m currently working on a project which require the conversion of a single stranded RNA virus genome (i.e. a norovirus) into a...

16 March 2021 1,999 2 View

Is it strictly recommended to run samples in duplicate during SNP genotyping analysis?

We are using AppliedBiosystems TaqMan® SNP Genotyping Assays. Is it ok not to run the samples in duplicate to increase sample size?

02 February 2021 9,110 3 View

Apricot database - where to search?

I work in apricot research and we have a breeding program for abiotic stress tolerance. I collect data from the cultivars i could use in this program individually, but there are several hundred...

01 February 2021 584 3 View

How can I get Prunus dasycarpa seeds?

For experimental work I would like to grow Prunus dasycarpa. Where can I find seeds?

13 January 2021 6,529 3 View

Can MaxEnt use background points with one or more missing environmental data?

I know that the allowpartialdata parameter, which is by default false, can be set to true, and in this case presence points with one or more (but not all) missing environmental data are not...

26 June 2020 464 0 View

Is there something I can do to increase the chances of successful ISSR bands amplification during PCR ?

Hello, I have a following issue: I extracted approx. 20 plant DNA samples from dry leaves (old herbarium items) using Qiagen DNeasy Plant Mini kit. When I run a PCR for housekeeping gene (alpha...

22 March 2020 2,842 6 View

Abcam anti-RBP4 and anti-Thy1 antibodies for brain tissues - any experiences?

Hey everyone! Have anyone used the rabbit anti-Rbp4 (ab109193) and rat anti-Thy1 (ab85352) antibodies for fluorescent immunoshitochemistry in mouse brain slices? Does anyone have any experience...

14 May 2019 1,429 0 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

28 July 2024 9,811 1 View

Hello Researchers, Can anyone guide me on estimating the Leaf Area Index (LAI) of wheat using only weather and soil parameters? Is it possible?

I need to estimate the LAI of a wheat crop using only weather and soil parameters. Can anyone please help me?

28 July 2024 4,886 2 View

What are the best practices for washing and preparing fruits and vegetables to reduce pesticide residues?

27 July 2024 9,147 4 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

How to extract binding energy from pv.maegz file without using Schrodinger?

I have conducted virtual screening using Schrödinger on a database of 17,000 molecules. Unfortunately, I cannot use the system with the Schrödinger license at the moment. I am trying to find a way...

18 July 2024 2,881 4 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

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17 July 2024 3,371 0 View

Any homemade protocol to extract genomic DNA from leaf litter?

We are trying to extract DNA from ant nests to amplify the 16S gene, but apparently, the extraction allows the passage of PCR inhibitors. Thank you for your comments

15 July 2024 9,314 2 View

How to Dedust Activated Carbon Granules?

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11 July 2024 9,782 2 View

Can type 2 diabetes be reversed at any age through proper diet and exercise?

Type 2 diabetes can be managed and, in some cases, put into remission through proper diet and exercise, regardless of age. Here are some key points: Weight Management: Achieving and maintaining a...

07 July 2024 8,745 2 View

Girma Hailemichael Gebre

Probably, you can develop a coefficient and with that, can be calculated.

Ákos Mendel

So You are saying, that probably it is not aviable for positive selection, but can be good for negative selection?

Andrew Paul McKenzie Pegman

You could do a correlation yourself. See:

Article Leaf area, final fruit weight and productivity in kiwifruit

Thank You Andrew! It is helpful. Any advice by comparing different cultivars?