Is it strictly recommended to run samples in duplicate during SNP genotyping analysis?

02 February 2021 8,877 3 View

Apricot database - where to search?

I work in apricot research and we have a breeding program for abiotic stress tolerance. I collect data from the cultivars i could use in this program individually, but there are several hundred...

01 February 2021 386 3 View

How can I get Prunus dasycarpa seeds?

For experimental work I would like to grow Prunus dasycarpa. Where can I find seeds?

13 January 2021 6,315 3 View

Is there something I can do to increase the chances of successful ISSR bands amplification during PCR ?

Hello, I have a following issue: I extracted approx. 20 plant DNA samples from dry leaves (old herbarium items) using Qiagen DNeasy Plant Mini kit. When I run a PCR for housekeeping gene (alpha...

22 March 2020 2,664 6 View

Are there any data available about the mechanism of loss of effectivity to immune checkpoint inhibitors?

Despite the good effectivity of the immune checkpoint inhibitors a proportion of patients will face relapse, and will be resistant to this treatment. I am intereseted if we know anything about...

03 January 2019 8,134 3 View

Do the AMV or M-MLV-RT (or derivatives) have any ligation or strand displacement activities?

21 October 2013 6,816 1 View

Bioclimatic variables: alternative way of selecting the specific month or quarter

Dear Species Distribution Modelers and All, Have you ever tried to select the specific month/quarter (e.g. wettest quarter for bio8) in a way other than the default one? If yes, how? If not, why...

01 January 1970 2,148 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

How can I import .sav file into LISREL?

I've just downloaded free trial version of LISREL 10.3 but I got stuck with importing .sav (IBM SPSS 25.) file. I mainly followed the steps of this video...

15 February 2021 752 2 View

Why is there cloudy white solution in flavonoid test tube?

I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...

08 February 2021 6,779 4 View

How to screen phytochemicals directly from the crude plant latex?

Number of methods are available to screen phytochemicals through extract prepared in different solvents including aqueous and organic. Is there any method/ published work related to phytochemical...

01 February 2021 7,348 4 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Apricot database - where to search?

I work in apricot research and we have a breeding program for abiotic stress tolerance. I collect data from the cultivars i could use in this program individually, but there are several hundred...

01 February 2021 386 3 View

How would you assess protein binding adjusted EC50 for a drug that has a binding rate of over 95%?

I'm in need of a reference or a method to come up with a protocol that allows one to get the protein-adjusted EC50 for an antiviral drug in presence of human serum albumin and acid glycoprotein.

27 January 2021 1,402 1 View

High throughput screen to identify drug induced cell death mechanism?

So I am working with a drug that we know induces cell death in MDA-MB-231 cells. In other cell lines we have been able to identify that this drug induces ferroptosis. I have also done experiment's...

25 January 2021 3,587 3 View