Dear all.
We are studying the pathophysiology og autoantibodies to GPCR that we induce in animal models. In addition to looking at the histological endpoints, we would like to purify the IgG from the animals and get dose-response curves for individual animals and compare, and if possible by price, charcterize their functionality (and not just titers we get) further. I have contacted several drug-development companies. They will do it, but at a steep price. Are there any academic GPCR service lab. that have analytical services? I have not been able to find one. We got nothing in-house and the time and sallary spendt developing will be much more then the drug-developing companies charge. Thanks for potential response.
Hi Lasse, can you provide more details on what type of functional assays you are interested at? Also, are you talking about polyklonal antibodies? In that case i have reservations about the utility of further functional studies. In addition, there are now good possibilities to isolate the human autoantibodies and study those. No need to involve induced animal antibodies which would be more prone to artefacts.
Thank you Markus!
I would like to look at activation of the second messenger, or any other version of confirmation of GPCR activation and ability to create a dose-response curve (some use arrestin, some use IC Calcium, (euroscreen/discoverX/eurofits). We want to look at anti-AT1R and anti-beta1.
Would love to use the human version, but we only have biobank material (With longitudinal data), so we dont want to spend alot of the serum, as its used by many other researchers.
We were going to use polyclonal antibodies, because we are looking at their ability to cause pathology and polyclonals more "Natural", even though raising them in Rabbits is far from ideal. The peptide immunization protocol is well known and has been used in several other studies. We want to get "dose-response" curves, as part of mapping their pathophysiological pontential.
Purification of the human forms will be a next step, but because of time/funding, we cannot do that now.
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies