Can anyone suggest a good method to polymerize "isopropylmethacrylamide"?

i need to polymerize the isopropylmethacrylamide monomers to get a polymer with LCST around 37C. i read in some articles which said it depends on polymer molecular weight.

04 May 2016 3,811 4 View

Could you introduce a cancer cell line with highly expressed P-glycoprotein?

i need a cancer cell line can express P-glycoproteins and also be susceptible to Doxorubicin.

03 April 2016 8,559 4 View

According to Poly N-vinyl caprolactam, what will happen on Nitrogen in its ring in an acidic environment(for example in Lysosome)?

if we want to compare this polymer versus polyvinylpyrrolidone, which one is better to use as a thermosensitive polymer capable to transport a hydrophobic drug to a cell?

01 February 2016 2,639 3 View

How can I calculate the acetylcholinesterase enzyme units?

i have some data about a recombinant enzyme and i want to calculate my enzyme unite. i have used the ellman method for enzyme activity test. ODfirst minite= 0.35 ODseconde minite=0.8 reaction...

04 May 2015 6,858 2 View

How can I clean the procainamid-spharose 4B from contaminant proteins?

I have a home made procainamide sepharose 4B column that completely filled by contaminant proteins. Can I use of EDTA as cleaning agent? Is it possible the EDTA destroy the procainamide?

02 March 2015 903 5 View

Is it possible to observe a the blue shift in UV-Vis spectroscopy after protein-nanoparticle incubation?

I am working on the interaction of silver nanoparticle in different size with acetylcholinestrase. i saw the blue shift in UV-Vis peak for treated nanoparticle with protein than blank one.

11 December 2014 2,444 7 View

Why does nanoparticle absorbance intensity increase after being treated by protein?

I treated the silver nanoparticle with acetylcholinestrase. data showed after 3 hours the maximum absorbance peak for nanoparticle-protein increased than blank one. in blank sample there is just...

11 December 2014 1,262 9 View

How can we separate the imidazole from a buffer?

07 August 2014 5,879 1 View

How can I separate Ag ions from Ag nanoparticles?

There are some references showing that we can use cellophane as a semipermeable membrane and electrophoresis.

03 April 2014 8,788 5 View

Why can abiotic stress have more of an effect on cell death than biotic stress?

Biotic stress such as viral infection.

02 March 2014 1,516 7 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View