How do you annotate figures to show significance?

30 January 2021 1,008 3 View

How can peptides based on allele sequences be delivered to mice?

I am looking to measure allele-specific immune responses for specific antigens. After identifying the most prominent alleles in a population, I am looking to express them in mice and then measure...

03 September 2020 9,281 1 View

Missing values in R lme(): how to include observations with missings in the outcome variable?

Hello, I am struggling to understand how I best use the na.action options in R's lme function for a hierarchical linear model. I have a longitudinal data set with a two-level nested structure...

23 July 2020 2,327 4 View

How do I interpret extremely low values of Blomberg's K with low P values?

I have a set of trees and am using Blomberg's K to detect phylogenetic signal. I am finding that for several of them, my values for K are close to 0 (in the region of K = 0.001 - 0.004) which...

05 December 2019 6,379 1 View

Warning messages in Abaqus relating to general contact, tie constraints, and stringer beam elements - are they an issue?

Hi, I have a 3D model that I am using to model a shear connections in reinforced concrete. The model is using C3D8R elements for the concrete and B31 elements for the reinforcement, which are...

27 February 2018 8,169 3 View

Liquid culture colony PCR technique?

I need to do a pcr on a liquid culture of E.coli. I'm amplifying a 150bp region of gyrA, using primers that I designed. I'm using NEB Next Ultra 2 Q5 MM. For the reaction, I am using 5ul NEB, 0.5...

25 October 2017 5,995 5 View

I am currently dealing with article critique on learning theories.? This been my first time to do this what would you recommend for effectiveness?

I am taking Edu 831 (Learning theories), it involves article critique on use of different learning theories in research. How can I critique an article effectively and what does it involve?

08 October 2017 1,958 3 View

How can research on attitude change benefit government initiatives to encourage young people to develop more healthy attitudes towards alcohol?

How do you think research on the Theory of Reasoned Action (TRA) and the Theory of Planned Behaviour (TPB) influence government initiatives to encourage healthy attitudes and perhaps even change...

12 August 2017 2,479 3 View

I would like to store 10 uL aliquots of bacterial culture. What would be the best way?

I need to store 10ul aliquots of bacterial culture , would the best way be to put them in PCR tubes with some gylcerol (10ul 50%) at -80?

31 January 2017 2,848 3 View

Unusual pelleting with ethanol precip after phenol chloroform. Help troubleshoot?

I've been using phenol chloroform extractions on my Chromatin IPs, but after the ethanol precipitation I either get very tiny pellets, or a smear along the side of the tube. I think it is...

07 June 2016 10,024 2 View

How I can extract pectinase from filamentus fungi? also get SDS-PAGE from it?

I am working on filamentus fungi, I culture it on broth culture and find the best time for extraction but still I cannot make sure that the way of extraction that I used is correct, actually I...

14 January 2021 4,150 3 View

Hemicellulose HPLC protocol?

I want to know the best solvent to characterize hemicellulose by high-performance liquid chromatography HPLC

11 January 2021 4,677 3 View

Any simplified protocol or methods of estimation of cellulose, hemicellulose and lignin content in a biomass?

Dear all, Kindly suggest me a simplified/easy method of calculation of cellulose, hemicellulose and lignin content of agricultural biomass along with the relevant reference. We have used the NREL...

02 December 2020 4,362 3 View

Could anybody help regarding MD simulation using AMBER?

Hi All, I have build the model of cellulose polymer using xleap and hemicellulose polymer using glycam carbohydrate builder. Now, I need to place the two polymer chain parallel two each other....

20 May 2020 9,416 1 View

What can be the presumable reason for the lower activity of cellulase at 5mM concentration of metal ions than at 2 &10mM concentration ?

I have purified enzymes cellulase and beta-glucosidase and performed SDS-PAGE. Therefore, I have characterized with metal ions at 2mM, 5mM and 10mM but at 5mM concentration lower activity than at...

25 March 2020 8,056 3 View

How are digestive enzymes for human consumption commercially produced?

Hello, Many protein shake powder mixes contain digestive enzymes such as protease, lipase, and cellulase. However, the products do not detail from which organism the proteins are produced or...

21 March 2020 5,048 3 View

How to convert the Fillter paper unit to G?

I need to perform enzymatic saccharification process for that i need to add cellulase enzyme. In the Ref paper everyone put as FPU. i dont know to convert FPU into mg. can anyone help with this?

23 February 2020 8,297 2 View

Suitable sample types for assaying cellulase activity?

Hello, I am trying to set up an enzymatic assay to determine the activity of cellulase. I would like to know what sample types would be suitable to perform the same? I have read certain papers...

01 February 2020 1,105 3 View

How to convert the Fillter paper unit to G?

I need to perform enzymatic saccharification process for that i need to add cellulase enzyme. In the Ref paper everyone put as FPU. i dont know to convert FPU into mg. can anyone help with this?

01 February 2020 8,283 2 View

How to create a standard curve of enzyme concentration and relative absorbance?

Hello, during an experiment I found myself in needing a standard curve of a kit for enzyme activity screening. The kit has a certain amount of substrate (cellulose) and I prepared different...

01 February 2020 2,552 3 View