How can I fix Pseudomonas putida cells prior coulter counter?

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Can some indicate a technique - or approach - to measure the thickness of a 20µm film and thickness with water uptake?

Hi, I need to measure the real-time (or temporal intervals) thickness variation of a transparent material from dry to wet/humid conditions, however, I cannot use the following techniques: -...

08 July 2024 4,799 4 View

THP-1 cells completely dying with PMA, help?

Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...

03 July 2024 3,884 5 View

Is there any situation where the use of negative q exponents in the formula of Hill numbers is justifiable?

Hill numbers have become an elegant and powerful unifying tool in the measurement of many levels of biological diversity. Although mathematically possible, the use of negative values for the...

09 June 2024 9,302 2 View

Is there any news on the treatment of macular injury from high-intensity LED light?

Hi everyone, I'm looking for papers regarding macular injury from LED light, but it seems there's only publications talking about acute lesions. Thanks in advance. Cheers!

13 May 2024 7,160 0 View

Can I purify large quantities of proteins using the ÄKTA protein purification system equipped with size exclusion or ion exchange columns?

Can I purify large quantities of phycocyanin (current concentration of 30%) using the ÄKTA system equipped with size exclusion or ion exchange columns? Could I perform multiple injections to...

26 March 2024 5,031 2 View

What is the most suitable conductivity cell (k=0.1; K=1) for evaluating the conductivity of pharmaceutical formulations such as liposomes, LNPs...?

Dear, What is the most suitable conductivity cell (k=0.1; K=1) for evaluating the conductivity of pharmaceutical formulations such as liposomes, LNPs...

24 March 2024 5,466 2 View

Barlat 1991 model. How are the parametes calculated?

I am doing a comparison between different anisotropy models. Now, I am understanding the Barlat 1991 model. I understand the concept but I have some doubts about how to proceed to calculate the...

10 March 2024 3,354 1 View

What are the best websites to find academic events?

I'm from Brazil, but I want to start proposing communications and papers for international events. Are there any specific websites I can use to find events? I really appreciate any help you can...

06 February 2024 2,489 1 View

Can Heavy metal pollution cause deffects in CaCO3 crystals from marine calcifiers?

My question is because we know that divalent ions can substitute calcium in the biomineralization process of some marine calcifiers and if we have a huge amount of free Fe2+ from pollution, for...

24 September 2023 2,697 1 View

Any suggestions for crab identification guides in sri lanka (estuarine/mangrove crabs)?

I am looking for some pictorial guides related to crab species in sri lanka. Any suggestions for such identification guides?

21 July 2023 1,800 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Arpan De

Ethanol:acetic acid(3:1) is one of the options but 2.5% Glutaraldehyde is certainly a better option.

Guido Lingua

Ethanol; Ethanol:acetic acid; Glutaraldehyde. Or you could try with microwaves: times and intensity will need to be optimized, but it is very fast and you don't need any chemicals.