I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to achieve the sensitivity diagram of a sensor in CST?

I wanna design a sensor in CST 2021 for the first time and I don't know how to achieve the sensitivity diagram of the sensor in CST. Could you plz help me to do that? I appreciate your response...

26 January 2021 6,864 1 View

What kinds of potential genes could be expressed in frogs exposed to chytrid fungus in different environmental conditions ?

I need an applicable molecular biomarker like expressing the genes in frogs who expose to chytrid fungus in different environmental stressors. Indeed, what kinds of genes I should notice in frog's...

25 January 2021 7,718 2 View

How to select the most non-correlated samples within a dateset?

Dear researchers How to select the most non-correlated samples within a dateset? Considering that the samples are time-series of length l within m different time-series. To more clarify the...

13 January 2021 741 6 View

DSC test- Glass transition temperature of the electrospun PA66 nanofibers?

Hello Everyone, I did several DSC tests on the electrospun PA66 nanofibers to obtain the glass transition temperature. According to the material datasheet, the Tg point of PA66, as cast, is around...

13 December 2020 1,506 2 View

How to create a sag and swell detection in the DVR applications?

I have created a three-phase cascaded h-bridge inverter at Simulink with the SHE-PWM technique, and I want to test it in the DVR applications. How can I design a controller for that in the DVR...

13 December 2020 735 3 View

Degenerated semiconductor (Burstein Moss effect) with unchanged band-gap?

Is it possible to produce a degenerate semiconductor using a dopant agent that results in increasing the electrical conductivity, but the optical band-gap remains unchanged? In such a situation...

11 December 2020 8,338 13 View

Which statistic software can give me a relationship for multivariate function?

Hi friend I have some data and they influence on each others and I draw their graph in excel but excel can't give me a relationship for multivariate function. the regression of these data in excel...

29 November 2020 9,371 9 View

A paper may be rejected because of several reasons?

In my case, I have received rejection after seven months from submission to journal without reason or editor comments (decline). Moreover, without being reviewed by an expert scientist reviewer,...

29 October 2020 5,437 4 View

Do scalar-tensor-vector gravity theories violate the Bekenstein-Hawking area law to compute the entropy of black holes?

I am looking for the thermodynamics of the cosmological black hole in scalar-tensor-vector gravity theories. I know how should I write and find the thermodynamical quantities and equations for...

26 September 2020 6,383 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

QTL, recombination frection?

22 February 2021 2,588 2 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Why the storage moduli E' decrease with highr fiber volume?

Hi guys, I am facing problems to interpret a DMA results. Material: Composite Bisphenol A + curaua fiber What is being tested: the influence of fiber volume on the composites properties...

12 February 2021 174 7 View

How reliable is estimation of copy numbers by absolute qPCR?

Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...

11 February 2021 8,421 13 View

Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View