There are several ways to do this, both experimentally and computationally.  Experimentally, you can run a native (non-denaturing) gel electrophoresis assay, or UV-crosslinking.  Computationally, you can use any of the software available in the internet that perform protein-protein or protein-ligand predictions.  HTH.

Thanks a lot to guide.

In addition to Vicente's recommendations, you can try affinity chromatography: crosslinking your protein to a resin using EDC etc. Proteins that bind your protein will remain in the column attached to the protein and can be eluted with chaotropic agents and analyzed by SDS-PAGE. Purified proteins can then be subjected to other assays/ techniques to ensure that the binding was not non-specific.

Got it. Thank you so much.