How can we choose the right method for Gaussian calculations?

I'm not working on biomolecules, I work on Organic, Organometallic, and sometimes inorganic structures. Do you know a simple way to apply the right calculation method? Is there any article or...

10 December 2020 3,396 5 View

How to dissolve sodium hyaluronate, 95% in water?

Hi, I am trying to dissolve Sodium hyaluronate, 95% in water with a concentration of 2.7% (w/v). However, it gets gel instead. So far, I have tried elevated temperature water bath (around 40...

30 August 2020 8,428 3 View

How we can find the extinction coefficient of antibodies?

I need extinction coefficient of anti-HER-2 antibody. where can I find it?

08 August 2019 9,276 1 View

I need a source about the different extraction methods and their effect on extraction of constituents in medicinal plants?

For example which method can extract polar and nonpolar constituents from medicinal plants easily?

07 May 2019 8,242 14 View

I have ordered some peptides and I need to add 5(6)-FAM to them. Anybody has any idea how i can do this?

These peptides are not on resin anymore and i do have Wang resin to maybe conjugate them back on the resin before attaching the dye but i am not quite sure how to do so! Is there a better way to...

11 March 2019 547 9 View

Sequence without ATGG?

24 August 2016 5,615 3 View

Why my breast cancer cell lines started to grow slowly and detach?

Does anyone has advices or explanation for the following? I have three cells line MCF-7, T47D and MDA231, when I received these cells in 25 cm flasks from friend's laboratory, they were adherent...

09 May 2014 7,125 11 View

DNA extraction of helico form biopsy

I faced with problem about extracting DNA of H.pylori directly form biopsy samples I am not sure about my method I am using CTAB-Chlrophorm method but i didnt get any result yet could you help...

16 July 2010 8,782 1 View

Is there anybody ,who have synthesized silica nanoparticles ?

for synthesizing silver-silica nanoparticles by sol-gel method , i have probem with it .

01 January 1970 3,708 8 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Does the phylogenetic tree making tool IQTREE use evolutionary placement algorithm?

For my master thesis I am conducting a phylogenetic research and for this I'm using tools like MEGA, IQTREE, FigTree, etc to make a phylogenetic tree. I wanted to know if IQTREE integrates the...

14 February 2021 1,541 2 View

Is there an alternative to PGLS that considers phylogenetic signal when calculating the estimated value?

I have been working on a study trying to estimate body mass in mammals using a skeletal proxy in R. My dataset has high phylogenetic signal (Pagel's Lambda = 0.9) and I am interested in removing...

26 January 2021 6,997 3 View

When making a phylogenetic tree, do I need to concatenate the chromosome and the plasmids for my reference?

This is such a basic question but I come from a non-bioinfo group so I keep getting different answers. I was wondering if when making a phylogenetic tree whether you have to concatenate the...

14 January 2021 2,492 2 View

How can I build one phylogenetic tree for an arthropod community when I had known their scientific names?

I am working on the phylogenetic analysis for communities of plants and arthropods. A standardized protocol of building phylogenetic tree for plant species list that was widely used in studies....

29 December 2020 181 3 View

Are phylogenetic independent contrasts still used in biology?

I have a regression equation in which I am curious if the amount of scatter around the regression line is driven by phylogeny. I know there is a lot of phylogenetic signal because Pascal's lambda...

22 December 2020 937 1 View

Could anybody provide insights the best tools to create a custom database for taxonomy assignment of metagenomic data?

I am interested in the different tools that can be used to create custom databases for targeted sequencing and how to trim the databases based on the amplicon size? Also, should custom databases...

02 December 2020 3,105 3 View

How can I get Genbank accession code given the scientific names?

Hello! I now have a complete list of about 23k plant species that I want to check if Genbank has the sequencing data for a phylogenetic analysis later, and I am really new to the topic. Is...

02 November 2020 4,450 3 View

Can you recommend me tree databases?

Hi. I have slight experience in searching phylogenetic trees, normaly I use TreeBASE or Dryad but some papers that I found doesn't have the resulting trees in these databases or there are genetic...

26 October 2020 582 3 View

When drawing a phylogenetic tree with somatic mutations(SNPs), what is the role of tree simulation process?

Hello, I am working on drawing a phylogenetic tree with samples sharing some somatic mutations. The basic process of my work is similar to this article. Population dynamics of normal human blood...

14 October 2020 8,078 4 View