I use the polyvinyl alcohol (eye-spear) sponge for collecting the cervicovaginal fluid. How such an extraction buffer can able to achieve extract all samples from sponge? (I will use in Elisa.) I found a couple of buffer formulas in some research, with PBS, NaCl, protease inhibitor cocktails, EDTA, Glycerol and sodium azide, etc. ... Any advice?
#cervicovaginal fluid #pva #sponge #extraction #buffer
Please look at the following below links which may help you in your analysis:
https://patentimages.storage.googleapis.com/bb/bd/6e/6ee568fd6fdd4e/GB2505557A.pdf
https://www.kinderkrebsinfo.de/patients/pohkinderkrebsinfountersuchungen/biopsies_and_punctures/index_eng.html
Thanks
John Carter Yes, I will centrifuge them. But this PVA material retain the content of liquid sample very closely. So, I must keep wait in the buffer and shake in the buffer to extract them, to recover them very well.
Isam Eldin Hussein Elgailani Thank you for your recommendation, but these protocols not related to my experiment unfortunately.
You could put the sponge in a plastic bag with ELISA buffer (PBST?) and squeeze it several times.
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