Dear Saraniya Bharathi,

Its somehow difficult to extract single protein from bio samples, because it's have verity of peptides/proteins in binding form in bio sample, when we starts to ectract it, it's nature was changed because its existing in bonded aggregated form, or with other compound. so that its difficult but it's possible.,

after remove possible unwanted things from sample, add TFA and wait for 5hrs and then you extract with diethyl ether, and by HPLC-LC-MS you cn able to separate the peptides., its common methods, for your concern i have send a msg some books and articles related to this.

Regards R.Selva.....

Hi Saraniya, hair, skin and nails is mostly dried and crosslinked keratin.

There are, however, antimicrobial peptides in these tissues. Is this your interest?

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664254/

http://yalma.ru/articles/Skin%20peptides-%20biological%20activity%20and%20therapeutic%20opportunities.pdf

Dear Saraniya Bharathi,

Its somehow difficult to extract single protein from bio samples, because it's have verity of peptides/proteins in binding form in bio sample, when we starts to ectract it, it's nature was changed because its existing in bonded aggregated form, or with other compound. so that its difficult but it's possible.,

after remove possible unwanted things from sample, add TFA and wait for 5hrs and then you extract with diethyl ether, and by HPLC-LC-MS you cn able to separate the peptides., its common methods, for your concern i have send a msg some books and articles related to this.

Regards R.Selva.....