How can I extract DNA from fleas?

More Carlos Alemán Dyer's questions See All

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

How do you delete a duplicate pdf for the same paper on ResearchGate?

The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.

07 August 2024 9,508 1 View

What does a decreasing 𝐶𝑒 vs. 𝑄e plot indicate in an adsorption isotherm?

I am conducting adsorption experiments in a liquid medium, and one of my samples showed a decrease in Qe values with an increase in Ce. Has anyone encountered this behavior before? What does it...

26 June 2024 7,304 2 View

Expert in advanced statistics interested in contributing to an academic article on the adoption of digital innovations in agriculture?

We are looking for a highly qualified researcher with expertise in advanced statistical analysis to contribute to a scientific article to be submitted to a prestigious journal by the end of the...

13 June 2024 569 4 View

Gel electrophoresis has 2 lanes with double bands, not possible?

I had to run a gel electrophoresis at college with an undigested pUC18 (all the way left), digested pUC18 (lane 7) and a digested version (lane 8) in which i inserted a fluorescent gene (pET...

23 May 2024 7,104 2 View

Word error in a title / full text (1 page) shared: may I correct it ?

A recently published visual abstract should have the title "Word First Myelography..." instead of the error "Mielography". (the 2 other text references in the article are correct) I am changing it...

20 May 2024 8,134 1 View

Fluorescence spectroscopy: signal from water?

In order to proceed with the quantification of an Azide Alkyne Cycloaddition reaction efficiency by using a fluorogenic probe with Cy5-DBCO, I first need to construct a calibration curve. The...

16 May 2024 6,266 5 View

Is there a commercially available cell line from human cancer-associated fibroblasts (CAFs) isolated from malignant pleural mesothelioma or lung ca ?

Dear community, I am currently working on a proposal focusing on cancer-associated fibroblasts (CAFs) from malignant pleural mesothelioma (MPM). In my search among commercial cell line providers,...

05 May 2024 5,572 4 View

Why I have less plasmid after the digestion with SmaI?

I'm performing a digestion with SmaI but when I concentrate the digested DNA with a Zymo Kit I obtain half of the inicial DNA. I'm working at 30°C like the insert says.

30 April 2024 8,673 0 View

For DOM (dissolved organic matter) and in addition to the Follin method strategy, is there another way to determine phenolic content in the sample?

This is working with DOM (dissolved organic matter) and in addition to the acid-base titration strategy, Follin's method, there is another strategy to determine the amount of phenolic?

23 April 2024 2,197 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Hudson Alves Pinto

Dear Carlos Dyer ,

There are several protocols on DNA extraction of fleas infected with Yersinia pestis available in the scientific literature. A few examples are presented in the attached papers. I hope they are useful for you.

Best regards,

Hudson

Hafiz Muhammad Ashraf

There are several protocols on DNA extraction. please try this protocol which are explained in this paper. link attached. this is easy and effective protocol. i hope this is helpful for you.

International Journal of Agriculture and Biology 07/2015; 17(4):809-814. DOI:10.17957/IJAB/14.0027