How can I extract cellular and secreted protein?

Saeid Afshar @Saeid-Afshar

08 September 2017 0 2K Report

I want to extract the secreted and cellular protein.

How can i perform it by lysate buffer?

Badges
Science topic

Similar topics
Mathematical Sciences
Graphs

More Saeid Afshar's questions See All

Surface adsorption with the help of iron and UV/H2O2 is innovative?

1.The topic I am going to research is "Advanced Gray Wastewater Treatment Output from the Biological Process by Iron-assisted surface adsorption and UV/H2O2". I think this method has been used a...

08 July 2024 9,464 1 View

How validate the dynamic molecualr simulation with experimental data?

I used forcite module of Material Studio software to simulate the dissolution of zinc production waste in deep eutectic solvent. UFF force field has been used for the optimization of molecules,...

02 July 2024 7,494 1 View

Is it possible to do simulation for laser ablation in water using COMSOL or Lumerical?

Solving heat transfer equation during laser ablation in air is done with comsol and there are some tutorial videos, is it possible to do do simulation for laser heating during ablation in liquid....

15 April 2024 9,515 1 View

In architectural design, should we start from inside or outside (society and external events, etc.)?

which one is more important in architecture design, internal subjects or external subject ? For example, are feelings and what we see more important or events such as society, politics,...

25 March 2024 610 16 View

Lumerical simulation help of silicon based avalanche photodiode with HgCdTe absorber material?

If someone can simulate it with Lumerical, I would like the attached items.

09 February 2024 8,905 2 View

dhb jhdcin cdjjncd ?

Fjkvchj

12 December 2023 224 1 View

Can architecture shape human thoughts and feelings?

According to the book " the eyes of the skin” by Juhani Pallasmaa , which says that the architect is in the architectural work, the question arises for me, if a person lives in this architectural...

05 December 2023 617 2 View

How is The Volume Expansion Coefficient for Low Solute Concentration Obtained?

Hello dear researchers, Do you know how to solve and prove the formula 5.27 (The Volume Expansion Coefficient for Low Solute Concentration) mentioned in the "diffusion mass transfer book" by...

18 November 2023 302 2 View

What software can be used to create architectural virtual environments?

I read in an article about virtual architectural environments and I was very eager to know where virtual architectural spaces can be built and used as galleries, classrooms, or work rooms,...

18 November 2023 1,401 2 View

Can artificial intelligence replace future architects?

With artificial progress, is there any need for designers and architects anymore? In your opinion, in which parts can artificial intelligence be replaced and in which part designers and...

11 November 2023 5,103 3 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

Why is serum-free media used when making cancer cell conditioned media?

I am preparing cancer cell conditioned media to study the effects of substances secreted by cancer cells on other cells. In some papers, conditioned media is prepared using FBS-containing media...

11 July 2024 7,063 1 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View