I have tried to digest pET 28a vector with BamHI and HindIII enzymes but didn't get any band rather very light multiple bands were appeared in the gel. How can I be able to double digest my vector as I am only restricted to use these enzymes.
YOU CAN
use the following protocol
Tango buffer 2 microL
MQ water 7.7 microL
DNA template 10 micro L
HINDIII 0.3-0.5 microL
BamHI 0.3-0.5 microL
AT 37 C for 2h and try at 37C for 40 minutes as well
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