How can I double digest pET 28a vector with BamHI and HindIII enzymes as their sites in vector are very close to each other?

I have tried to digest pET 28a vector with BamHI and HindIII enzymes but didn't get any band rather very light multiple bands were appeared in the gel. How can I be able to double digest my vector...

05 May 2018 1,656 1 View