How can I do nonreversible chemical nuclease inactivation in bacterial cells and keep DNA intact?

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Can you recommend a short and free Deep Learning course that is sufficient to enable implementation on Java?

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

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fatal error

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fatal error

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Geoff Margison

Please clarify: do you want to maintain the E.coli as intact cells while you inhibit nucleases within the cells? If so, I have no idea! However, iIf you are disrupting the cells, use a high concentration of EDTA (I have seen up to 50millimolar being used in some protocols). This chelates especially magnesium ions that are essential for the action of the vast majority of enzymic nucleases. IF you really do mean "chemical nuclease" you need to be more specific.

Vladimir N Loparev

EDTA not working,

I tried.

Absolutely great paper - thank you Andrei!!!! For your opinion what is the best inhibitor there ??