Nuclear Magnetic Resonance ( NMR) usually conducted in solid or semi solid stage. It is better to provide sample in solid state as to get molecular structure.
Nuclear Magnetic Resonance ( NMR) usually conducted in solid or semi solid stage. It is better to provide sample in solid state as to get molecular structure.
Hi Virendra,
I think the NMR machine in our lab is only for liquid, so they dissolve the solid biomass and use it as solution in NMR machine
any suggestions?
Thanks
Hi Ahmed
Can you suggest me with which chemical or method I have to dissolve the solid biomass because my sample of biomass(i.e. recycled sludge) is not getting dissolve in water. and I need to do its NMR and HPLC?
If you only have access to a solution state NMR instrument, you'll definitely need to dissolve it, and youll ONLY see signals from the dissolved components, nothing else. You can try different solvents to dissolve different components of your biomass, or extract the components with different solvents. You'll have to do solid state NMR for the insoluble components. Only way to do all of this together in one step without modifying the sample is to use a multiphase probe (Bruker).
I think this paper will be very useful for you:
Elucidating Structural Characteristics of Biomass using Solution-State 2 D NMR with a Mixture of Deuterated Dimethylsulfoxide and Hexamethylphosphoramide. The best choice for biomass is an instrument for liquid state,
Hi Akansha Pandey,
There are different solvent that can do the job, please see the attached review about the routes of biomass dissolution and what is the suitable solvent
Hope this helping
Thanks
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