I'm trying to micropropagate a very slow growing plant and thought of providing CO2 to accelerate its growth. I do not have any "special" device or equipment to facilitate the introduction of CO2 into the culture vessels. Any DIY suggestions?
I have used (sodium) bicarbonate to mimic/ simulate CO2 enrichment (http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144206) and many others have done so. Easy to add at some stage of growth or into suspension cultures, but on agar may be tricky. Because if you bubble CO2 from a cylinder into agar media before cooling and buffer to resist pH changes, after cooling it may have different pH and CO2 levels would change (unsure how it would diffuse or not). Another way to grow them in Petridishes in a CO2 incubator that animal cell culture people, if you can (but they usually lack light, which you can install!). Thanks, Biswa
sodium bicarbonate in a glass at the base of tray and some acetic acid drop added daily would generate enough co2 to keep a volume of 5 l above 1000 vpm. a film enclosure may be necessary but autotrophy requires also the lowering of media sugars.
Thank you for the suggestions, Dr. Biswapriya Biswavas Misra ·and Dr. Guido Bongi!
- Badges
- Science topic
- Similar topics
- Agriculture
- Horticulture
- Seeds