I harvested a virus. When I did plaque assay to titer it, I did duplicate and the titer was different. How can I take one of them? Will I average them? The concentrations were 4.50E+05 and 2.50E+05 PFU/ml.
I will do a PRNT test with these virus. Therefore, It is important to know its titer as accurately as possible! Any help would be greatly appreciated!
If an accurate measurement is important, maybe it would be best to repeat the measurement a few more times since the two values you got are so different. Is your solution homogenous / properly suspended / well mixed?
Repeat the assay and maintain replicates (3 or more) to get near by values. As Laura Leighton suggested previously, make sure that reagents and samples are mixed properly for better homogenization.
When you did the plaque assay did you run in duplicates? Also as titers can vary for a virus (i.e. mixing, pipetting, some viruses are sticky) its a good idea to at least repeat the assay 3 times and then use the average from all three. Even better would be to get someone else to repeat it as well 3 times. If the virus has been titered before by your lab it would be good to compare results. The variation you have is not a big difference but it depends on what the titer is being used for.
Thank you, All for your valuable comments. I really appreciate your responses greatly. I did one more round of plaque assay today. Let's see what comes up!
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