I have serum samples collected by someone else. I am not sure samples collected properly that's why I want to check their quality before doing an ELISA assay. Is there any validated spectrophotometric method for detecting serum quality? or should ı check each sample for hemolytic (H), icteric (I), and lipemic (L) disturbances?
You are going to use your samples for ELISA test. Thus, I propose you to make a pool of some trusted serum samples and compare the total protein content of your suspected samples with the pool. I guess this is a simple and effective way.
Dear Duygu,
That is really an important issue but unfortunately difficult.
Since you are working with sera in ELISA, I suppose your aim is to detect antibodies! If I am right with that, as a simple way, I advice you to test your target/s in ELISA and compare results with another test targeting the same target.
In general antibodies are stable for years if kept at -80 degree Celsius. I have been using human sera since 2007 and still working for antibodies.
Hi Duygu, You did not tell the purpose of the serum. I guess for antibodies detection. I suggest you check the antibody titres with already known antigens in your lab and compare then only go for your new test. Usually, antibodies retain in the samples years after collection if stored properly. 10 to 15 years is common.
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