How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

How to calculate the sample size required to detect the difference in pharmacokinetics of methotrexate?

I came across with this study here, https://www.clinexprheumatol.org/abstract.asp?a=5066 And this study says: "The sample size calculation was done to compare two different MTX starting dose...

14 February 2021 3,017 3 View

How to calculate ultraviolet dose in Gy or J/kg?

Currently I'm doing a literature review regarding the comparisons of different types of radiation such as uv, electron beam and gamma radiation. But I'm having a difficult time converting uv unit...

11 February 2021 7,642 3 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

Does someone know which structure could exit the olfactory bulbs at their antero-dorsal top ?

Hi people, I am studying bat brains through endocranial casts and I cannot identify which structure could be present in sort of a canal located dorsally to the cribriform plate. It is like...

27 January 2021 797 6 View

What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

How to improve my BrdU immunostaining?

24 January 2021 414 3 View

Is there a study on the maximum thumb extension that can be possible in a range of population while using injectors?

In Most pen injectors while dialing the dose, the dose dialer comes our or extends outside the pen as dialing happens. How much maximum extension of dialer is acceptable for performing...

17 January 2021 9,770 1 View