I injected the h-AMSC-luciferase (10 6 ) into the mouse model of hepatocellular carcinoma in situ by tail vein. At the beginning, the h-AMSC can be detected in the lung by the small animal imaging systerm (2 hours after injection), but after 30 hours nothing could be detected! I wanted to know whether the h-AMSC could homing to the tumor that inplanted in the liver. Is anyone had been did this experment? Or give me some suggestion!
As mesenchymal stem cells have a natural tendency to home at the site of injury one should be able to detect the labeled MSCs injected through the tail vein homed into the damaged organ.
As control experiments, I could suggest you make some injury in the liver and then inject the labeled cells through the tail vein. It is more likely to see the homing of the labeled MSCs in the liver.
Secondly, if the tumor was implanted in the liver sometime ago, and the injury has healed there are chances that you will not be able to detect any transplanted cells in the liver.
Thank you for doctor bipasha and Daniel. I still have some questions, first, after 30th hour I can't detect any fluorescence in the body, just the in the vein tail where I injected the AMSC had little signal be detected. So is it mean that AMSC is still alive, but it disperse to the every part of the body, then it caused the signal is so weak that it can't be detected? And some weeks later they will homing to the tumor and be detected?The second, I had executed the mouse and found the tumor was inplanted in liver and caused some necrosis in the liver, is it enough to recruit the AMSC to the liver?
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