06 December 2016 2 10K Report

Hi everyone,

Now, I'm starting to make my project, it is extracting ccfDNA from blood by THP protocol (Triton, heat, PCI). I really want to know about simple methods to detect infection gDNA into ccfDNA. Can anyone give me some suggestions?

Thanks for your help.

More Ngan Nguyen's questions See All
Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Any suggestion on self-efficacy scale ?

Dear researchers, I am planning on conducting a research on the relationship between Language learning strategies, by Oxford (1990) and self-efficacy. I have found Morgan-Jinks Student Efficacy...

22 January 2021 8,432 3 View

I need to apply a questionnaire from an article. What statistical analysis do I need to use?

Hello, I found a survey about attitude toward language in an article which I find fit to use it in my thesis. The survey has 30 questions, which were put into 5 factors through factor analysis...

21 January 2021 9,419 3 View

Why did I obtain abnormal staining by TUNEL assay?

I use Abcam TUNEL Assay Kit - HRP-DAB (ab206386) on mouse tissues. The staining showed a pattern that I expected to see with a specific layer of cells dying due to drug treatment. But the...

12 January 2021 144 1 View

Is there a generalized method to derive current-mode model of any DC-DC converters?

Hi all, As new DC-DC converters are being proposed, associated control method should be proposed and based on the small-model signal analysis of the DC-DC converters. Using average space state...

06 January 2021 7,247 3 View

Is it need to test Cronbach's Alpha for extracted features from data?

As Cronbach's Alpha is usually used for test the reliability of measurements given by questionnaires, however, I wonder that whether I use this test for features extracted from behavioral data. I...

26 December 2020 9,602 2 View

How is datasets good?

Can you show me some ways to generate benchmark datasets?

22 December 2020 362 4 View

Does crystallinity degree, (and crystalline phase) of a polymer affect its surface energy and adhesion?

I have several PLLA electrospun nanofibers with different crystallinity degrees and also different crystalline phases and I am measuring the adhesion force by AFM-based techniques. I wonder if we...

28 November 2020 3,269 5 View

Similar questions and discussions
MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close