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Hi everybody, I am using the High Pure Viral Nucleic Acid Kit (Roche) for extraction genomic DNA from blood (buffy coat). The DNA concentration is quite good (50-100 ng/ul) and the A260/A280 ratio...
04 September 2018 3,436 4 View
Hi everyone, Last week, I used the kit to purify a linear plasmid after digest with PCR purification protocol, but just obtained low DNA yield (2 ug linear plasmid input and only 250 ng for...
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Hi everyone, I've performed binding cells on gold surface through thiol SAM using aptamer for a month. But the result is not good, cells do not bind tightly on gold surface, they can be removed...
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Hi everyone, I am a student conducting a research experiment about ccfDNA from plasma, so I want to separate plasma from whole blood without breaking down leucocytes. I've read that to separate...
09 January 2017 6,270 4 View
Hi everyone, Now, I'm starting to make my project, it is extracting ccfDNA from blood by THP protocol (Triton, heat, PCI). I really want to know about simple methods to detect infection gDNA into...
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Hi everybody, I wonder that if I should resuspend biotin-labeled primer in nuclease-free water because I afraid that it may affect to oligo stability when store long-term. So which is...
01 January 1970 6,225 3 View
Dear all, I am working about lateral flow assay. Everything has been good until the last few days, after I treated the conjugate pad, I applied antibody-gold conjugate on to the pad and dry at...
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