Can I use a normal CO2 incubator with some modification ?
I will really appreciate your help.
Hello Leonor Huerta
You can try the method used in the research article below.
A simple new method for exposing cells to hypoxic conditions would be to use vacuum bags, normally employed for food storage, to establish and maintain low oxygen levels in vitro.
Please refer to the article attached for more details on how to create a low oxygen environment using vacuum bags.
Article An effective, low-cost method for achieving and maintaining ...
However, this new system is not suitable for experiments that require continuous monitoring of physiological parameters, for example during the period of switching from normoxia to hypoxia. Time-course experiments can be performed easily using samples in separate bags that are harvested at different time points. If the incubation condition needs to be changed during the process of hypoxia, for example to add agonists or antagonists at different times, re-gassing would be required. The vacuum bag system is most suitable for experiments where all additions to the medium are made at time 0 and measurements made only at one time point.
Hope this helps.
Best.
Wowww !! This is great. I have those bags and pump in my kitchen.
I'll check the article carefully.
Thank yoy very much!