Enzyme assays of mutant GBA cell lines ?

How do i measure the enzyme activity of human GBA in cos 7 cells that have been transfected with mutant plamids. After 24 hours of plating cells in a 12 well plate, I transfect cos 7 cells with...

30 August 2020 4,496 3 View

What are the samples that shows positive confirmation for ninhydrin test?

Is we have any raw sample extract that show positive confirmation in ninhydrin test?

12 February 2019 4,636 6 View

What were the main plant morphological characters considered during molecular phylogenetic studies?

Plant Morphology versus Molecular phylogeny

08 October 2017 2,328 9 View

How the dioecy in plants limits its breeding and cultivation ?

25 July 2017 9,463 3 View

How the sex of an uni-sexual / Dioecious plant changed by the application of Auxin hormone ?

Is this method was applying in Horticulture or Agro-forestry systems ? Is this have any connection with physiological stress or evolution ?

04 July 2017 2,520 8 View

Why a morphological data appeared in-congruent with all molecular data( sequenced data )during phylogenetic analysis ?

Reasons behind the in-congruence in plant genera based on taxonomy and evolution?

26 June 2017 6,742 3 View

Can anyone suggest some classical plant phylogenomics research papers for review?

21 June 2017 4,685 3 View

What were the phytogeographical elements on speciation in plants?

Attach some journal papers listing the phytogeographical elements leads to speciation and endemism in plants?

25 May 2017 6,897 3 View

How can we analyse the wood quality of a plant experimentally?

23 February 2017 9,131 1 View

Why should we do cloning for phylogenetic markers before sequencing ?

12 February 2017 546 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View