I've been working with a Chequerboard method, wich combines one antibiotic and one efflux pump inhibitor. Through that assay, i can determine the FIC index, wich avaliates the interaction between those two drugs. I would like to create a graphic representation of those results using 1MIC, 1/2MIC, 1/4MIC, 1/8MIC of the drugs alone and combined to make it easier to explain my results.
Does anyone know a software capable of that?
Hi!
Did you ever figure out how to create graphs for your drugs alone and in combination? I am in the same situation that you were once in and I would greatly appreciate your help.
Thanks,
Alysha
Hi Alysha!
I'm sorry to say it, but not yet :/. But one of colleagues knows someone who could help. We have yet to arrange a meeting, but once a learn how i coukd help you.
Keep in touch!
Hi Alysha. I've learned how to do the graph. Do you still need help?
Get in touch!
Hi, i need your help how to construct isobologram to check synergism between two antimicrobial drugs. I have the data obtained by checkerborad method. can you help me please?
Hello @Muhammad.
The model I was able to come up is not a "robust" one. I mean, it is not done by a specific software.. It is just a simple excell file which could generate a curve (Isobologram). If you would like me to send you the file either way, just tell me.
Hi, could you send me the file that you mentioned before? I need your help to create isobologram, thank you
Hello, Joao.
I am trying to make the same type of analysis but with biofiolm information from a checkerboard assay. Do you mind sharing with me the excel file? Thank you!
Hello all.
I will add the file and a quick description of my thoughts behind it:
-To explain briefly, at our lab we work with M. tuberculosis, which has a slow rate of growth. To solve this problem, we use Resazurin (colorant agent), to reveal the assay plate post incubation. The change from blue, to pink indicates bacterial growth. In this Excel File, there is an image of a plate that I created as an example of how to apply the data. The Blue wells are water (used to keep the plate from drying, once we have to incubate for 7 days); the pink wells are the ones that showed bacterial growth; the orange ones are the ones that showed synergistic effect (when the combination of the two drugs was able to kill the bacteria). Therefore, I checked the concentration of each drug used in each marked well (orange) to plot the data... Which gave me data to apply in a Dispersion graph, considering the drug A (antibiotic in my case) being in the Y-axis, and drug B in the X-axis. Additionally, I’ve used the MIC of drug A and B to create a dotted line which would tell me that any point that is on top of the line does not have a synergic interaction. Points below the line indicate synergism and points above the line indicate antagonism. I've applied a line of tendency, that can predict, based upon the results I’ve plotted, other concentrations that could show the same effect. I don’t know if I was clear enough… If you have any doubts, you can ask me, and I’ll try to help you out. It wasn’t easy for me to “learn” how to do it, since I haven’t found any paper that explained step by step how to do it. I’ll wait for your response. PS: if you change the data according to your experiment, the graph will adapt.
To create the best isobolograms you should plot fractional MIC data of both drugs on x and y axis.
Zineddine Benbekhti Please send me a personal message. I can try to help you.
João, a Mariana Terron usou alguns softwares para encontrar o MIC...converse com ela.
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