João Vítor Perez de Souza

3 Questions 9 Answers 0 Followers

Questions related from João Vítor Perez de Souza

Is there a way to freeze infected THP-1 "macrophages" before extracting RNA?

Hello everybody. Does anyone know if I could freeze my infected THP-1 macrophages before extracting RNA? The reason to do so would be that I would like to perform controls to check if the cell...

30 May 2018 9,864 2 View

How long does RNA remain viable at -80ºC freezing?

I'm planning to extract the RNA from my bacterial cultures , but the reagents for the Reverse Transcriptase haven't arrived yet. How long could I keep the RNA frozen?

19 April 2017 2,735 6 View

How can i create a graphic representation (isobologram) of several MICs obtained from a Chequerboard method?

I've been working with a Chequerboard method, wich combines one antibiotic and one efflux pump inhibitor. Through that assay, i can determine the FIC index, wich avaliates the interaction between...

09 May 2016 2,543 12 View