João Vítor Perez de Souza

5 Questions 10 Answers 0 Followers

Questions related from João Vítor Perez de Souza

Is there a way to freeze infected THP-1 "macrophages" before extracting RNA?

Hello everybody. Does anyone know if I could freeze my infected THP-1 macrophages before extracting RNA? The reason to do so would be that I would like to perform controls to check if the cell...

30 May 2018 9,874 2 View

THP-1 culture doubts

Hello everyone, I am starting to culture THP-1 cells but I need some guidance. I am sorry or all the newbie questions, but the former student who started the culture in our lab, left without...

06 July 2017 9,097 7 View

How long does RNA remain viable at -80ºC freezing?

I'm planning to extract the RNA from my bacterial cultures , but the reagents for the Reverse Transcriptase haven't arrived yet. How long could I keep the RNA frozen?

19 April 2017 2,744 6 View

How can i create a graphic representation (isobologram) of several MICs obtained from a Chequerboard method?

I've been working with a Chequerboard method, wich combines one antibiotic and one efflux pump inhibitor. Through that assay, i can determine the FIC index, wich avaliates the interaction between...

09 May 2016 2,548 12 View

Is it possible to use Compusyn software to analyse synergism between 2 drugs on in vitro Chequerboard method?

I've been working on a Chequerboard method on a 96-well plate, wich combine one antibiotic and one efflux pump inhibitor.Through the Chequerboard method itself, i can calculate the FIC index, wich...

09 May 2016 2,829 0 View