7 Questions 12 Answers 0 Followers
Questions related from João Vítor Perez de Souza
We hope to produce a recombinant mycobacterial RNA polymerase through heterologous expression in E. coli to evaluate the activity of some drug candidates as RNAP inhibitors. Unfortunately, up...
18 February 2019 8,349 4 View
Hello everybody. Does anyone know if I could freeze my infected THP-1 macrophages before extracting RNA? The reason to do so would be that I would like to perform controls to check if the cell...
30 May 2018 9,922 2 View
Hello! I am currently trying to apply PMA treatment in THP-1 cells to enable differentiation to macrophage-like cells but I am not sure about their morphology. Could anyone with more expertise...
18 July 2017 4,467 3 View
Hello everyone, I am starting to culture THP-1 cells but I need some guidance. I am sorry or all the newbie questions, but the former student who started the culture in our lab, left without...
06 July 2017 9,122 7 View
I'm planning to extract the RNA from my bacterial cultures , but the reagents for the Reverse Transcriptase haven't arrived yet. How long could I keep the RNA frozen?
19 April 2017 2,780 6 View
I've been working with a Chequerboard method, wich combines one antibiotic and one efflux pump inhibitor. Through that assay, i can determine the FIC index, wich avaliates the interaction between...
09 May 2016 2,576 12 View
I've been working on a Chequerboard method on a 96-well plate, wich combine one antibiotic and one efflux pump inhibitor.Through the Chequerboard method itself, i can calculate the FIC index, wich...
09 May 2016 2,858 0 View
