We strongly believe that our treatment is causing cell proliferation after looking at insulin content and total protein. I wanted to quantify DNA with either DAPI or Hoechst staining. Is there a way to determine cell number with a plate reader, or is a flow cytometer required? Also, is fixing required, or can you quantify on lysed cells?
The easiest and cheapest way to do this, though the most painful probably, is to plate the cells onto glass coverslips, treat, fix and DAPI stain. Then you can mount of coverslip and count nuclei under a scope. This will give you an absolute number cells that you have for certain. Flow cytometry is easier but it costs money and you need equipment. Fixed cells are easiest and you can count all the cells including control quickly if you have one of those clickers that counts everytime you click.
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