How can I convert the Agarose Gel image for publication?

More Anandharaj Marimuthu's questions See All

How to design guideRNAs that should target all the alleles of a gene in monocot?

In banana, we are having multiple alleles. For distrusting the function of a gene through CRISPR/Cas9 it is important to mutate all the alleles through designing specific guideRNA repeats. How to...

15 August 2022 5,313 1 View

How to do Fluid-structure Interaction modelling of patient specific 3D arterial blood flow?

I am getting convergence issues in the first coupling iteration itself and the solver stops. I tried refining the mesh and also tried reducing the time step size. It is still not working. If...

06 July 2020 6,617 7 View

Influence of COVID on the mental health - Survey?

Dear Pls, follow the link provided below to register to invaluable experiences in the face of COVID-19. https://swinuw.au1.qualtrics.com/jfe/form/SV_88qs1TRRQAv8Q9T

14 April 2020 384 3 View

Can anyone say about to make a perfect way to make GO bases Nanocomposite?

I am working in ternary composites. Is there anyone working in graphene and its derivatives. I want to make a composite comprised of GO with some inorganic material.

05 May 2019 6,977 3 View

How to model the leakage of water from a closed container when the valve is opened?

I patched the fluid domain with 1400 gauge pressure and set up the boundary condition at the outlet to be 0 gauge pressure. But I am getting floating point exception. What am i missing?

04 April 2019 8,579 4 View

Can anyone say clearly about the calculation for doping metal oxides using some different mol ratios?

I need to dope metal to my metal oxide matrix, Can anyone clearly explain me about the calculations that i need to workout for mol %.

26 March 2019 1,470 4 View

Is there any medium to support the growth of Streptomycetes only?

Does the growth of microbes on ISP4 medium confirm the organisms to be a streptomycete? Kindly suggest some selection medium for Streptomycetes

17 February 2019 7,336 3 View

Where can can I buy Ceramic Matrix Composites (CMC) SiC-C or SiC-SiC?

Hi, Does any company in Europe sell Ceramic Matrix Composites (CMC) (SiC-C or SiC-SiC) components or flat plates. Thanks Sundar

04 February 2019 4,467 3 View

Reference required - reg?

Dear Friends, In Biodiversity Heritage Library, I couldn't download the volumes from 8 onwards. If anybody is having the following reference, kindly support me to share it. "Clark AM 1967. Notes...

02 January 2019 6,975 6 View

Air pollutant accumulated Filter paper preservation?

Usually the filter papers will be desiccated for 24 hr before and after ambient air pollutant sampling. As we encounter some error in weighing balance, we are unable to weigh the exposed air...

22 October 2018 1,925 4 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

19 July 2024 148 6 View

Less substrate signals towards the last lanes of the Native PAGE?

I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...

17 July 2024 6,213 4 View

Help. How can I fix my T7 reaction problems?

I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...

16 July 2024 4,604 0 View

Can anyone help in getting software related to gel scoring of silver stained PAGE for diversity study?

I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...

12 July 2024 7,145 2 View

How much 1X TAE buffer should I use in preparing 1.2% agarose gel?

Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...

12 July 2024 2,058 5 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

Ying Henderson

Do you have "invert display" button on your gel image system? If you are using Bio-Rad, they have this function.

Jacques Davy Ibaba

Which gel imaging system do you use ? you don't need to change the background the journal editor request it.

Christopher J Gillson

Easily done in The Gimp, free image software. Load your black background image in and press invert.

If you have Adobe Photoshop, you can save your image as tiff file and open in Photoshop. Under "Layers", click "invert" under "refine", your image will have white background.

Hui Wang

I'd like to know if it is allowed to put an "invert display" gel picture on publicated paper?