Influence of COVID on the mental health - Survey?

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14 April 2020 191 3 View

Can anyone say about to make a perfect way to make GO bases Nanocomposite?

I am working in ternary composites. Is there anyone working in graphene and its derivatives. I want to make a composite comprised of GO with some inorganic material.

05 May 2019 6,642 3 View

Can anyone say clearly about the calculation for doping metal oxides using some different mol ratios?

I need to dope metal to my metal oxide matrix, Can anyone clearly explain me about the calculations that i need to workout for mol %.

26 March 2019 1,277 4 View

Is there any medium to support the growth of Streptomycetes only?

Does the growth of microbes on ISP4 medium confirm the organisms to be a streptomycete? Kindly suggest some selection medium for Streptomycetes

17 February 2019 7,129 3 View

Air pollutant accumulated Filter paper preservation?

Usually the filter papers will be desiccated for 24 hr before and after ambient air pollutant sampling. As we encounter some error in weighing balance, we are unable to weigh the exposed air...

22 October 2018 1,718 4 View

What is the reason of decrease the bandgap when decrease the cystallite size?

I prepared nanorod structured undoped (pure) oxide thin film by spray pyrolysis method function of different substrate temperatures (STs). I found that decrease the bandgap when decreases the...

08 August 2017 5,868 21 View

Which online software is reliable for Protein Transmembrane helix prediction

25 May 2017 6,484 4 View

What are the existing "Quality Assessment Models" available for building or general construction?

Similar to CONQUAS (Singapore) and PASS (HK), Is there any other quality assessement models available in the practice or in the literature domain?. Thanks Marimuthu K

26 October 2016 6,584 4 View

Double band for a single protein on western blot?

Im working on yeast secretary protein, my protein was his tagged so i use Anti-his monoclonal antibody for WB, i can see the signal but i got two band on each protein (totally i have two proteins...

16 December 2015 5,351 12 View

How to select a correct primer for an unknown organism for PCR?

We isolated bacteria from various samples. It is identified by biochemical tests as Lactobacillus spp. Now we are going to sequencing it for species level identification. I'm a little confused...

29 March 2014 1,563 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View